Slanting mullarkey

[barkar]

Read the article , seems like i have a lot to do if i am going to use this Conan Yeast to its full potential. How many slants could one get out of the pitch pack ? Would ye pitch into new wort  or just innoculate , where would ye get the necessary gear , seem some  cryo tubes on ebay fairly cheap,
Must procure a pressure cooker some where.....

[Hop Bomb]

Pitch most of the pack in a brew & save a little bit for slanting in a clean & sterile container in the fridge. HBW have cryo tubes. Inoculate one or all of the cryo tubes with the bit of yeast you saved if you want (think there 12-15 pearls in each tube) then freeze those tubes.

Then you can make a slant from one pearl when you've all your kit together (you can borrow my pressure cooker - or do what tube does & microwave them) Then when that one slant takes off you can inoculate more slants from that one slant.

[barkar]

Would freezing the yeast not kill off the cells , sorry starting this from a very knowledge deficient starting point on this !I was contemplating doing a starter to build up to a 40 litre brew , put some pearls in a cryo tube and then use for slants ?

[Hop Bomb]

No cell damage if you freeze them in the cryo tubes. Its got glycol solution in there or similar.

[johnrm]

Are we slanting or freezing here?
Freezing with glycol is a different process.
There was a mention of the coldest party of the fridge, not the freezer.

[Hop Bomb]

Are we slanting or freezing here?

Both. Cryo tubes for freezing the cells for long term storage. Then make one slant from one pearl - when that grows you can inoculate more slants & store them in the fridge for your yeast starters.

[johnrm]

So Freeze the Agar slants then?

[Hop Bomb]

No. Yer freezing some yeast in the cryo tubes. Then you remove a pearl or two of yeast from these to make slants as you need them.



Someone should really do a youtube video or guide as there is zero info on it anywhere. Even the instructions with the cryo tubes are a bit vague.

[barkar]

They seem like the easier option as i dont have all the gear yet. So drop 25 pearls into a tube and freeze . Then when have gear for slanting take 'em out and put 1 pearl per slant so effectively you have 25 long term usuable pitches from each cryotube ? Sounds good   

[Hop Bomb]

The cryo tubes have 25 pearls in each tube. You get 4 tubes for 15e in HBW.

You put some yeast in the tube, close the lid & shake it up with the solution thats in there & then freeze the tube.
Then you can just take 1 pearl out & make one slant. When that slant grows a few days later you can inoculate more
slants from that slant. Im guessing you could get maybe 10 slants from 1 pearl. So 250 slants from one cryo tube.
Then add in reusing yeast from brews & you'll probably never run out.

[Shane Phelan]

Someone should really do a youtube video or guide as there is zero info on it anywhere. Even the instructions with the cryo tubes are a bit vague.

Would you be interested in doing a small guide on the cryo tubes as while slanting is fine for medium term, I find your experience with the cryo would be useful information to brewers for the long term?

[johnrm]

Sorry, the subject was slanting and I thought referred to the recent (and super) slanting how to.
Should we do similar on Cryo?

[Shane Phelan]

It definitely seems to be a viable option.

[Hop Bomb]

There isnt much to it really. Just chuck in some yeast in the cryo tube - shake it - freeze it. Then when you want to slant just take out a pearl, inoculate your agar in the slant & let it grow. Then a few days later you make slants off that slant instead of a vial of white labs for example.

[barkar]

Someone should really do a youtube video or guide as there is zero info on it anywhere. Even the instructions with the cryo tubes are a bit vague.

Would you be interested in doing a small guide on the cryo tubes as while slanting is fine for medium term, I find your experience with the cryo would be useful information to brewers for the long term?

Looks like i will be calling in to HBW in the next week !