National Homebrew Club Ireland

Brewing Discussions => All Grain Brewing => Topic started by: Shane Phelan on April 15, 2013, 10:27:11 AM

Title: Want to know what bacteria is in the air when bottling?
Post by: Shane Phelan on April 15, 2013, 10:27:11 AM
I was telling a few people about this at the brewday in TOG yesterday. One of the guys that I work with was analysing how much bacteria fell in a given area (Petri dish) over a 90 minute period. He gave everyone in my lab a dish to try at home to expose for 90minutes.

I decided to expose mine while I was bottling beer as sometimes it is exposed for a while when siphoning and while in the bottling bucket. Here is a pic of what fell after 4 days in an incubator at 26 degrees.

(http://img407.imageshack.us/img407/7380/img20130415095617.jpg)

Some of it is mould and the rest is mainly caused by "skin flora (http://en.wikipedia.org/wiki/Skin_flora)" which are common to every household. One of the science girls is going to analyse it in a bacterial database later to get a specific list of the species. 

The contamination concentration is proportional to the amount of airflow in the apartment/house. So any open vents, doors, fans etc will contribute more bacteria to the sample. As I was bottling I was moving around a fair bit, opening doors, washing bottles, carrying boxes in addtion to two PC's running with fans in both. So I see my sample as a kind of worst case scenario for my apartment....hopefully... ;D Definitely recommended keeping your beer covered while cooling!
Title: Re: Want to know what bacteria is in the air when bottling?
Post by: Will_D on April 15, 2013, 11:15:24 AM
Thats amazing! What particular nutient agar was that grown on?

Wouldn't mind some in the next GB!
Title: Re: Want to know what bacteria is in the air when bottling?
Post by: Shane Phelan on April 15, 2013, 11:24:14 AM
I think its a general purpose agar mix. They also have other mixes specifically for growing yeast and other organisms but those types wasn't used for this test. All I can tell you is that it stinks and smells like science lab.  ;D
Title: Re: Want to know what bacteria is in the air when bottling?
Post by: imark on April 19, 2013, 12:31:46 PM
Wonder what and how much of each species is in there? Will we know if this could have any effect on the end taste of the bottled beer?
Title: Re: Want to know what bacteria is in the air when bottling?
Post by: Shane Phelan on April 28, 2013, 06:41:40 PM
These are a couple of 'blank' slants from the TOG brew day a few weeks ago. I decided to see what would happen if I made slants from vials that had not been sanitised. I think they were open a few times. The first one is mould and the second looks to be more of that "skin flora" that was showing up on the plate earlier in the thread.
(http://img15.imageshack.us/img15/3733/img1043ra.jpg)
Title: Re: Want to know what bacteria is in the air when bottling?
Post by: Tom on April 28, 2013, 06:43:13 PM
Mmm... skin flora.
Title: Re: Want to know what bacteria is in the air when bottling?
Post by: LordEoin on April 28, 2013, 10:49:03 PM
I thought the crabs ate all that kinda stuff.... ::)