Recommendations for a reasonably priced microscope

[Shanna]

Hi there,

Am toying with the idea of buying a microscope for home use. Want something that would have strong enough magnification to be able to use with a haemocytometer for yeast counting as well as for mounting and viewing slides

Any assistance on what to look out for would be appreciated. Budget up to €500.

Shanna

[Thewicklowhopscompany]

Hi,
For counting cells you do not really need too much magnification or an insanely expensive microscope. Yeast cells are pretty big, something like this would do the trick.
https://www.amazon.co.uk/Apex-Microscopes-The-Practitioner-Microscope/dp/B001BPQL4C/ref=sr_1_6?ie=UTF8&qid=1534279634&sr=8-6&keywords=microscope

I would recommend a decent hemocytomoter like this one:
https://www.amazon.co.uk/Counting-Improved-Neubauer-Hemacytometer-MarienFeld/dp/B073BH852J/ref=sr_1_8?ie=UTF8&qid=1534279824&sr=8-8&keywords=Hemocytometer

Then all you need is some sterile water for diluting and a couple of pipettes.
https://www.amazon.co.uk/3ML-GRADUATED-PASTEUR-PIPETTE-PK10/dp/B009WQPXLE/ref=sr_1_5?s=industrial&ie=UTF8&qid=1534279876&sr=1-5&keywords=pipettes

These for loading the chamber
https://www.amazon.co.uk/Disposable-Micropipette-Pipette-Pipettor-Tipes/dp/B01EZZQKL8/ref=sr_1_6?s=industrial&ie=UTF8&qid=1534279932&sr=1-6&keywords=fine+tip+pipettes

Also if your interested in testing the viability you can pick up methylene blue solution. The methylene stains the dead/not viable cells.  Give me a pm if you get the stuff and I can give you a few tips.


Phil

[Shanna]

Cheers Phil will look at them appreciate the links.

Shanna

Hi,
For counting cells you do not really need too much magnification or an insanely expensive microscope. Yeast cells are pretty big, something like this would do the trick.
https://www.amazon.co.uk/Apex-Microscopes-The-Practitioner-Microscope/dp/B001BPQL4C/ref=sr_1_6?ie=UTF8&qid=1534279634&sr=8-6&keywords=microscope

I would recommend a decent hemocytomoter like this one:
https://www.amazon.co.uk/Counting-Improved-Neubauer-Hemacytometer-MarienFeld/dp/B073BH852J/ref=sr_1_8?ie=UTF8&qid=1534279824&sr=8-8&keywords=Hemocytometer

Then all you need is some sterile water for diluting and a couple of pipettes.
https://www.amazon.co.uk/3ML-GRADUATED-PASTEUR-PIPETTE-PK10/dp/B009WQPXLE/ref=sr_1_5?s=industrial&ie=UTF8&qid=1534279876&sr=1-5&keywords=pipettes

These for loading the chamber
https://www.amazon.co.uk/Disposable-Micropipette-Pipette-Pipettor-Tipes/dp/B01EZZQKL8/ref=sr_1_6?s=industrial&ie=UTF8&qid=1534279932&sr=1-6&keywords=fine+tip+pipettes

Also if your interested in testing the viability you can pick up methylene blue solution. The methylene stains the dead/not viable cells.  Give me a pm if you get the stuff and I can give you a few tips.


Phil

[garciaBernal]

https://www.gtvision.co.uk/Aspen-GTC10-Trinocular-Biological-Microscope-with-LED-Illumination

I picked up this one for less than 500 through lads called Trafalgar Scientific in the UK. You'll need to email them and haggle a bit.
I wanted one with oil immersion as I'm analysing a lot of wild yeast so wanted to get deep down into it but if you got a 500 budget I recommend getting one with oil immersion.

[Will_D]

 That reminds me Mr Dempsey!!

[DEMPSEY]

That reminds me Mr Dempsey!!

I know,I was only thinking of contacting you the other day about dropping it back.